Qualitative analysis of a sample using an algorithm
AssigneeRoche Molecular Systems, Inc.
InventorRolf Knobel
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Abstract
To determine the presence of a specific nucleic acid sequence within a sample, a labelled substance, capable of binding the nucleic acid to be determined, or a labelling substance is added, the substance having the ability to label the nucleic acid or being representative for the nucleic acid. The nucleic acid is amplified, the presence of which is to be determined and the increase of the labelled substance and/or the effect initiated by the labelled substance due to the increase of this specific nucleic acid is determined. The signal increase and/or the effect against time is analysed using a model for determining a deviation from a linear curve.
Claims6
Method for determining the presence of a specific nucleic acid within a sample comprising adding a labelled substance capable of binding to the nucleic acid to be determined or a labelling substance, the labelling substance having the ability to label the nucleic acid or being representative for the nucleic acid, amplifying the nucleic acid, the presence of which is to be determined, measuring the change of a signal initiated by the labelled substance or the labelling substance due to the increase of the specific nucleic acid, and analysing the signal change initiated by the labelled substance or the labelling substance against time using a model for determining a deviation from a linear curve according the following formula:
- f(x)=β1+β2·x+β3·s(x),
- where β1 and β2 are the coefficients for the linear curve and β3 is the coefficient for a preset nonlinear shape s(x).
Dependencies are inferred from the claim text.