Cyclic peptides and antibodies thereof
OwnerJames Tam, Chadler Pool +2inventors
InventorsJames Tam, Chadler Pool, Ying Zhang, Kristen Sadler
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Abstract
The present invention provides methods of preparing and identifying antibodies against a loop domain of a protein, such as an extracellular loop (ECL) domain of a transmembrane protein. Cyclic and end-to-end cyclized peptides corresponding to loop domains are employed in the present invention. Transmembrane proteins contemplated by the invention include the G-coupled protein receptor or a viral envelope protein.
Claims40
A method for preparing an antibody against a protein loop domain comprising:
- (a) providing a cyclic peptide corresponding to said protein loop domain;
- (b) administering said cyclic peptide to an experimental animal under conditions supporting production of antibodies;
- and
- (c) obtaining from said animal (i) an antibody that binds to said protein loop domain or (ii) an antibody-producing cell, antibodies from which bind to said protein loop domain.
A method for identifying an antibody against a loop domain of a protein comprising:
- (a) providing a cyclic peptide corresponding to said loop domain;
- (b) contacting said cyclic peptide with a population of antibodies;
- and
- (c) identifying an antibody that binds to said loop domain.
An end-to-end cyclized peptide corresponding to a loop domain of a protein.
A composition comprising a plurality of end-to-end cyclized peptides corresponding to a plurality of loop domains of a protein.
Dependencies are inferred from the claim text.