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US 2006/0068422 A1Published application
Gene chip technology for determining memory genes
AssigneesCold Spring Harbor Laboratory, Emory University, Hoffmann-La Roche Inc.
InventorsTimothy Tully, Joshua Dubnau, Michael Davis, Jan Mous, Ulrich Certa
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Abstract
The present invention relates to methods of identifying genes involved in memory formation. This is accomplished by performing a gene chip identification of those genes expressed during transcription-dependent memory formation but not during transcription-independent memory formation. A statistical analysis of the gene chip identification output yields a set of genes that are involved in transcription-dependent memory formation.
Claims26
A method of identifying a gene or genes involved in transcription-dependent memory comprising the steps of:
- (a) training non-human animals under conditions sufficient to induce transcription-dependent memory formation in said animals;
- (b) extracting RNA from brain tissue of said animals trained in step (a);
- (c) synthesizing DNA probes using the RNA extracted in step (b);
- (d) exposing the DNA probes synthesized in step (c) to microarray chips containing DNA sequences from genes of the genome of said animals under conditions appropriate for hybridization of the DNA probes to complementary DNA sequences on the microarray chips, wherein a signal is produced upon hybridization of said probes to complementary DNA sequences;
- (e) detecting the signal produced in step (d);
- and
- (f) performing a statistical comparison between the signal detected in step (e) and the signal detected in a control, wherein said control is obtained according to a method comprising the steps of:
- (i) training non-human control animals under appropriate conditions, wherein said conditions are insufficient to induce transcription-dependent memory formation in said control animals;
- (ii) extracting RNA from brain tissue of said control animals trained in step (f)(i);
- (iii) synthesizing DNA probes using the RNA extracted in step (f)(ii);
- and
- (iv) exposing the DNA probes synthesized in step (f)(iii) to microarray chips containing DNA sequences from genes of the genome of control animals under conditions appropriate for hybridization of the DNA probes to complementary DNA sequences on the microarray chips, wherein a signal is produced upon hybridization of said probes to complementary DNA sequences.
9 more elements
A method of identifying a gene or genes involved in transcription-dependent memory comprising the steps of:
- (a) training Drosophila under conditions appropriate to induce transcription-dependent memory formation in said Drosophila;
- (b) extracting RNA from head tissue of Drosophila trained in step (a);
- (c) synthesizing DNA probes using the RNA extracted in step (b);
- (d) exposing the DNA probes synthesized in step (c) to microarray chips containing DNA sequences from genes of the Drosophila genome under conditions appropriate for hybridization of the DNA probes to complementary DNA sequences on the microarray chips, wherein a signal is produced upon hybridization of said probes to complementary DNA sequences;
- (e) detecting the signal produced in step (d);
- and
- (f) performing a statistical comparison between the signal detected in step (e) and the signal detected in a control, wherein said control is obtained according to a method comprising the steps of:
- (i) training control Drosophila under appropriate conditions, wherein said conditions are insufficient to induce transcription-dependent memory formation in said control Drosophila;
- (ii) extracting RNA from head tissue of said control Drosophila trained in step (f)(i);
- (iii) synthesizing DNA probes using the RNA extracted in step (f)(ii);
- and
- (iv) exposing the DNA probes synthesized in step (f)(iii) to microarray chips containing DNA sequences from genes of the Drosophila genome under conditions appropriate for hybridization of the DNA probes to complementary DNA sequences on the microarray chips, wherein a signal is produced upon hybridization of said probes to complementary DNA sequences.
9 more elements
A method of identifying a gene or genes involved in transcription-dependent memory comprising the steps of:
- (a) training non-human animals under conditions sufficient to induce transcription-dependent memory formation in said animals;
- (b) extracting RNA from brain tissue of said animals trained in step (a);
- (c) synthesizing DNA probes using the RNA extracted in step (b);
- (d) exposing the DNA probes synthesized in step (c) to microarray chips containing DNA sequences from genes of the genome of said animals under conditions appropriate for hybridization of the DNA probes to complementary DNA sequences on the microarray chips, wherein a signal is produced upon hybridization of said probes to complementary DNA sequences;
- (e) detecting the signal produced in step (d);
- and
- (f) performing a statistical comparison between the signal detected in step (e) and the signal detected in a control, wherein said control is obtained according to a method comprising the steps of:
- (i) extracting RNA from brain tissue of non-human control animals;
- (ii) synthesizing DNA probes using the RNA extracted in step (f)(i);
- and
- (iii) exposing the DNA probes synthesized in step (f)(ii) to microarray chips containing DNA sequences from genes of the genome of control animals under conditions appropriate for hybridization of the DNA probes to complementary DNA sequences on the microarray chips, wherein a signal is produced upon hybridization of said probes to complementary DNA sequences.
8 more elements
A method of identifying a gene or genes involved in transcription-dependent memory comprising the steps of:
- (a) training Drosophila under conditions appropriate to induce transcription-dependent memory formation in said Drosophila;
- (b) extracting RNA from head tissue of Drosophila trained in step (a);
- (c) synthesizing DNA probes using the RNA extracted in step (b);
- (d) exposing the DNA probes synthesized in step (c) to microarray chips containing DNA sequences from genes of the Drosophila genome under conditions appropriate for hybridization of the DNA probes to complementary DNA sequences on the microarray chips, wherein a signal is produced upon hybridization of said probes to complementary DNA sequences;
- (e) detecting the signal produced in step (d);
- and
- (f) performing a statistical comparison between the signal detected in step (e) and the signal detected in a control, wherein said control is obtained according to a method comprising the steps of:
- (i) extracting RNA from head tissue of control Drosophila;
- (ii) synthesizing DNA probes using the RNA extracted in step (f)(i);
- and
- (iii) exposing the DNA probes synthesized in step (f)(ii) to microarray chips containing DNA sequences from genes of the Drosophila genome under conditions appropriate for hybridization of the DNA probes to complementary DNA sequences on the microarray chips, wherein a signal is produced upon hybridization of said probes to complementary DNA sequences.
8 more elements
Dependencies are inferred from the claim text.