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US 2006/0068419 A1Published application
Detection of nucleic acids to assess risk for bovine spongiform encephalopathy
AssigneeChronix Biomedical
InventorsEkkehard Schuetz, Leonid Iakoubov, Howard Urnovitz
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Abstract
The present invention provides a method of detecting abnormal serum nucleic acid profiles to assess the risk of a transmissible spongiform encephalopathy, e.g., BSE.
Claims20
A method of detecting an animal at increased risk for bovine spongiform encephalopathy (BSE), the method comprising:
- incubating nucleic acids extracted from an acellular sample obtained from the animal with amplification primers in a test amplification reaction;
- detecting reactivity of the amplification reaction that is over 3 standard deviations from a reference amplification reaction, wherein reactivity of over 3 is indicative of an increased risk for BSE.
A kit comprising primers that hybridize to the same sequences as the primers CHX-1F and CHX-1R.
A method of identifying primers to use in an amplification reaction to detect at animal at increased risk for bovine spongiform encephalopathy (BSE), the method comprising:
- identifying nucleic acid sequences that are enriched in BSE animals compared to normal;
- designing primers based on the sequences that are enriched in BSE;
- and
- selecting primers that detect reactivity in an amplification reaction comprising nucleic acids from a cohort or BSE animal that is over 3 standard deviations from a reference amplification reaction
1 more element
Dependencies are inferred from the claim text.