Long oligonucleotide arrays
AssigneeClontech Laboratories, Inc.
InventorsAlex Chenchik, Alexander Munishkin, Peter Simonenko
Life of this patent
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Abstract
Long oligonucleotide arrays, as well as methods for their preparation and use in hybridization assays, are provided. The subject arrays are characterized in that at least a portion of the probes of the array, and usually all of the probes of the array, are long oligonucleotides, e.g. oligonucleotides having a length of from about 50 to 120 nt. Each long oligonucleotide probe on the array is preferably chosen to exhibit substantially the same high target binding efficiency and substantially the same low non-specific binding under conditions in which the array is employed. The subject arrays find use in a number of different applications, e.g. differential gene expression analysis.
Claims13
-23. (canceled)
A method of preparing an array comprising at least one pattern of probe oligonucleotide spots stably associated with the surface of a solid support, wherein each probe oligonucleotide spot corresponds to a target nucleic acid and comprises an oligonucleotide probe composition made up of long oligonucleotide probes ranging in length from about 50 to 120 nt, said method comprising:
- generating said long oligonucleotide probes;
- and
- stably associating said long oligonucleotide probes on the surface of said solid support in a manner sufficient to produce said array.
A hybridization assay comprising the steps of:
- contacting at least one labeled target nucleic acid sample with an array according to claim 1 under conditions sufficient to produce a hybridization pattern;
- and
- detecting said hybridization pattern.
(canceled)
Dependencies are inferred from the claim text.