Methylated promoters of colon cancer-specific expression-decreased genes and use thereof

A primer mixture containing at least one primer pair selected from the group consisting of SEQ ID NOs:

• 17/18, SEQ ID NOs:
• 19/20, SEQ ID NOs:
• 21/22, SEQ ID NOs:
• 23/24, SEQ ID NOs:
• 25/26, SEQ ID NOs:
• 27/28, SEQ ID NOs:
• 29/30, and SEQ ID NOs:
• 31/32.

A method for detecting promoter methylation of a clinical sample-derived gene, the method comprising the steps of:

• (a) isolating sample DNAs from a clinical sample;
• (b) treating the isolated sample DNAs with (i) an agent that modifies nonmethylated cytosine residues, or (ii) any one or more methylation sensitive restriction enzyme selected from the group consisting of HpaII, MspI, BssHII, BstUI and NotI;
• (c) amplifying the treated DNAs using a primer capable of amplifying CpG island, which is originated from the promoter of the gene selected from the group consisting of LAMA2, FABP4, GSTA2, STMN2, NR4A2, DSCR1L1, A2M and SEPP1;
• and
• (d) determining promoter methylation according to the presence of the amplified product in the step (c).