Immuno polymerase chain reaction assay

An immuno polymerase chain reaction method of detecting at least one biological molecule in an assay sample, said method comprising:

• i) providing a preparation comprising a ligand:oligonucleotide conjugate and an assay sample, wherein said ligand binds said biological molecule to be detected in said assay sample;
• ii) adding a preparation comprising a single stranded template DNA molecule which anneals to said oligonucleotide in (i), deoxynucleotide triphosphates, and a DNA polymerase that elongates the primed oligonucleotide to produce a double stranded template DNA molecule;
• and
• iii) amplifying said double stranded template DNA molecule with a polymerase chain reaction which detects said template DNA molecule using oligonucleotide primers adapted to amplify said double stranded template DNA molecule.

A method of detecting at least one biological molecule in a sample to be tested, said method comprising:

• i) providing a preparation comprising;
• a) an assay sample to be tested;
• and
• b) a ligand:oligonucleotide conjugate;
• ii) incubating said preparation under conditions which allow the binding of said conjugate to said biological molecule to form a complex;
• iii) contacting the complex formed in (ii) with a single stranded DNA template nucleic acid adapted to anneal to a part of the oligonucleotide in said conjugate;
• iv) providing a DNA polymerase which is capable of elongating said single stranded DNA template to form a double stranded DNA template and amplifying said double stranded DNA template in a polymerase chain reaction wherein oligonucleotide primers used in said amplification comprise a palindromic sequence.