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OwnerSatoru Iizuka, Toshiyuki Takai +3inventors
InventorsSatoru Iizuka, Toshiyuki Takai, Yumi Ito, Kozue Ito, Masuo Obinata
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The present invention provides an immortalized natural killer cell line retaining the function and characteristics intrinsic to natural killer cells, a method for establishing the same, a method for screening for useful substances using the immortalized natural killer cell line, and a cell vaccine. By culturing natural killer cells obtained by isolating natural killer cells from the spleen of a transgenic mouse to which a large T-antigen gene of SV40 temperature-sensitive mutant tsA58 is introduced, a cell line which proliferates and activates in the presence of Interleukin-2, has azurophilic granules within cytoplasm, and retains an ability to kill a target cell without presensitization and/or an ability to kill target cells coated with an antibody, is established.
An immortalized natural killer cell line derived from spleen cells that proliferates and activates in the presence of Interleukin-2, has azurophilic granules within cytoplasm, and retains an ability to kill a target cell without presensitization and/or an ability to kill a target cells coated with an antibody.
An immortalized natural killer cell line selected from the group consisting of immortalized natural killer cell line TNK1 (FERM BP-08518), immortalized natural killer cell line TNK2 (FERM BP-08519), immortalized natural killer cell line TNK3 (FERM BP-08520), immortalized natural killer cell line TNK4 (FERM BP-08521), immortalized natural killer cell line TNK6 (FERM BP-08522), immortalized natural killer cell line TNK8 (FERM BP-08523), immortalized natural killer cell line TNK9 (FERM BP-08524), immortalized natural killer cell line TNK10 (FERM BP-08525) and mmortalized natural killer cell line TNKb (FERM BP-08526).
A method for producing immortalized natural killer cell line comprising culturing natural killer cells obtained from spleen of a transgenic mouse, in which a large T-antigen gene of SV40 temperature-sensitive mutant tsA58 is introduced, and by establishing a cell line that proliferates and activates in the presence of interleukin-2, having azurophilic granules within cytoplasm, and retaining an ability to kill a target cell without presensitization, and/or an ability to kill a target cell coated with an antibody.
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