Methods and materials for optimization of electronic transportation and hybridization reactions
AssigneeNanogen, Inc.
InventorsDaniel Smolko, Paul Swanson, Dalibor Hodko, David Canter, Robert Haigis, Tricia Patterson
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Abstract
Methods for the transport and hybridization of a nucleic acid on an electrode device by providing a low conductivity buffer with a reducing agent to the device. The low conductivity buffer may also contain a zwitterion. A current and voltage is applied to a location of the device to effect electrophoretic transportation of the nucleic acid towards the location. The nucleic acid is then hybridized to a nucleic probed located at the location. The reducing agent in the low conductivity buffer may also be acting as a chaotropic agent.
Claims21
A method for the transport of a nucleic acid to an electrode site comprising the steps of
- providing a low conductivity, zwitterionic buffer with a reducing agent to the active electronic system;
- applying current and voltage to an electrode to effect electrophoretic transportation of the nucleic acid towards the electrode.
21-107. (canceled)
Dependencies are inferred from the claim text.