Stem cell and progenitor cell expansion
AssigneeDuke University
InventorsTannishtha Reya, Mweia Uqoezwa, Qiju Wu, Leah DiMascio
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Abstract
The present invention provides compositions and methods for the expansion of stem cells and progenitor cells with adiponectin, adiponectin variants, or other molecules that activate adiponectin receptors and signaling, whereby the stem or progenitor cells retain their pluripotential phenotype after expansion. In certain embodiments, the present invention provides adiponectin-expanded stem cells used to treat disease (e.g. used in bone marrow transplants) or allows direct delivery in vivo to enhance hematopoietic recovery.
Claims20
A method for in vitro expansion of stem cells comprising:
- a) providing:
- i) stem cells;
- and
- ii) a composition comprising adiponectin or an adiponectin variant;
- and
- b) culturing said stem cells with said composition under conditions that promote expansion of said stem cells.
A composition comprising;
- a) ex vivo sorted stem cells;
- and
- b) adiponectin or an adiponectin variant present in a concentration between 50 and 150 ng/ml.
A kit comprising;
- a) a composition comprising adiponectin or an adiponectin variant;
- and
- b) written instructions for using said composition to expand stem cells.
Dependencies are inferred from the claim text.