US 2006/0054511 A1 · Electrochemical deblocking using a hydrazine derivative

Abstract

A method for electrochemical removal of acid-labile protecting groups on an electrode microarray using an organic solution is disclosed. The solution comprises a hydrazine derivative and a salt in an organic solvent. The hydrazine derivative has at least one hydrazine group having at least one hydrogen. The hydrazine derivative provides acidic reagent when an electrode is active and isolates the acidic reagent to the area around the active electrode. The salt is an organic salt or ionic liquid having a concentration sufficient to provide electrochemical conductivity under an applied voltage. During the applied voltage, acidic reagent is generated, which removes acid-labile protecting groups thereby allowing continued addition of monomers to build a custom microarray of oligonucleotides, peptides, or other polymers.

Claims19

Dependent claims

A method for electrochemical deblocking of an acid or base-labile protecting group, comprising applying a voltage or a current to at least one electrode of an array of electrodes, wherein the array of electrodes is covered by a deblocking solution, wherein the deblocking solution comprises an organic solvent and:

(a) a salt, whereby the salt provides conductivity to the deblocking solution;
and
(b) a hydrazine derivative, wherein the hydrazine derivative comprises at least one hydrazine group having at least one hydrogen atom attached to the at least one hydrazine group, wherein the hydrazine derivative provides an acidic or basic electrochemically-generated reagent, and the hydrazine derivative substantially isolating the acidic or basic reagent to a non-overlapping area surrounding the at least one electrode, whereby the electrochemical deblocking occurs when the acidic or basic reagent removes the at least one acid or base-labile protecting group at the at least one electrode.

A deblocking formulation used to selectively cause electrochemical deblocking at activated electrode sites on an electrode array having a plurality of separate electrodes, comprising:

(a) an organic solvent;
(b) a salt, whereby the salt provides conductivity to the deblocking solution;
and
(c) a hydrazine derivative, wherein the hydrazine derivative comprises at least one hydrazine group having at least one hydrogen atom attached to the at least one hydrazine group, wherein the hydrazine derivative provides an acidic or basic electrochemically-generated reagent, and provides electrochemical and the hydrazine derivative substantially isolating the acidic or basic reagent to a non-overlapping area surrounding an electrode.

1 more element

Dependencies are inferred from the claim text.

Electrochemical deblocking using a hydrazine derivative

Life of this patent

Electrochemical deblocking using a hydrazine derivative

Life of this patent

Abstract

Claims19