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AssigneeThe University of Hong Kong
InventorsMee Chye, Hong Li, Sathiskumar Ramalingam, Leo Poon, Joseph Peiris
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The invention relates to genetically modified plants and progeny thereof which constitute edible plant-derived mucosal vaccines and injectable plant-derived mucosal vaccines against Severe Acute Respiratory Syndrome (SARS). The invention relates to a recombinant vector that transforms specifically, but not limited to, the nuclei and/or plastids of tobacco, tomato and lettuce plants for antigen production. In specific embodiments, the plastid transformation vector expressing the nucleotide sequences are pCV1, pCV6 and pCV8, and their derivatives containing an S1 with nucleotide (but not amino acid) changes to optimize codon usage for expression in plants. The present invention relates to methods of making the modified plants which comprises transformation of plants with vectors for nuclear expression and/or plastid expression of nucleotide sequences of the SARS-CoV virus, fragments, derivatives, analogs, or variants thereof. The present invention also relates to methods of immunization against SARS and methods of antibody detection using the SARS-CoV antigens generated by the plastid and/or nuclear vector(s) transformed plants.
A vector comprising an isolated nucleic acid which nucleic acid sequence is set forth in SEQ ID NO:708 or 709, or a fragment, derivative or analog thereof.
A plant cell comprising an isolated nucleic acid which nucleic acid sequence is set forth in SEQ ID NO:708, 709, or a fragment, derivative or analog thereof.
A method of immunization against SARS comprising administering to a subject a plant, wherein the plant comprises an isolated nucleic acid which nucleic acid sequence is set forth in SEQ ID NO:708, 709, or a fragment, derivative, analog, or vector thereof.
A plant comprising the isolated nucleic acid which nucleic acid sequence is set forth in SEQ ID NO:708, 709, or a fragment, derivative or analog thereof.
Dependencies are inferred from the claim text.