Method for producing ketocarotenoids by cultivating genetically modified organisms
OwnerSabine Steiger, Gerhard Sandmanninventors
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Abstract
The present invention relates to a process for preparing ketocarotenoids by cultivation of genetically modified organisms which, compared with the wild type, have a modified ketolase activity, to the genetically modified organisms, and to the use thereof as human and animal foods and for producing ketocarotenoid extracts.
Claims43
A process for preparing ketocarotenoids by cultivating genetically modified organisms which, compared with the wild type, have a modified ketolase activity, and the modified ketolase activity is caused by a ketolase comprising the amino acid sequence SEQ ID NO:
- 2 or a sequence which is derived from this sequence by substitution, insertion or deletion of amino acids and which has an identity of at least 42% at the amino acid level with the sequence SEQ ID NO:
- 2.
A genetically modified organism where the genetic modification
- A, in the case where the wild-type organism already has a ketolase activity, increases the activity of a ketolase compared with the wild type and
- B, in the case where the wild-type organism has no ketolase activity, causes the activity of a ketolase compared with the wild type,
- and the ketolase activity which has been increased as in A or caused as in B is caused by a ketolase comprising the amino acid sequence SEQ ID NO:
- 2 or a sequence which is derived from this sequence by substitution, insertion or deletion of amino acids and which has an identity of at least 42% at the amino acid level with the sequence SEQ ID NO:
- 2.
A genetically modified organism comprising at least one transgenic nucleic acid encoding a ketolase comprising the amino acid sequence SEQ ID NO:
- 2 or a sequence which is derived from this sequence by substitution, insertion or deletion of amino acids and which has an identity of at least 42% at the amino acid level with the sequence SEQ ID NO:
- 2.
A genetically modified organism comprising at least two endogenous nucleic acids encoding a ketolase comprising the amino acid sequence SEQ ID NO:
- 2 or a sequence which is derived from this sequence by substitution, insertion or deletion of amino acids and which has an identity of at least 42% at the amino acid level with the sequence SEQ ID NO:
- 2.
A ketolase comprising the amino acid sequence SEQ. ID. NO. SEQ ID NO:
- 2 or a sequence which is derived from this sequence by substitution, insertion or deletion of amino acids and which has an identity of at least 70% at the amino acid level with the sequence SEQ ID NO:
- 2, with the proviso that the amino acid sequence SEQ ID NO:
- 2 is not present.
A ketolase comprising the amino acid sequence SEQ ID NO:
- 4 or a sequence which is derived from this sequence by substitution, insertion or deletion of amino acids and which has an identity of at least 70% at the amino acid level with the sequence SEQ ID NO:
- 4.
(canceled)
A ketolase comprising the amino acid sequence SEQ ID NO:
- 4 or a sequence which is derived from this sequence by substitution, insertion or deletion of amino acids and which has an identity of at least 65% at the amino acid level with the sequence SEQ ID NO:
- 4.
Dependencies are inferred from the claim text.