Splice variants of peptide YY, neuropeptide Y, pancreatic peptide Y and Amylin, and uses thereof

An isolated chimeric polypeptide, comprising a first amino acid sequence being at least about 90% homologous to amino acids 1-63 of known preproPYY (SEQ ID NO:65), and a second amino acid sequence being at least about 85% homologous to amino acids 91-97 of known preproPYY (SEQ ID NO:65), wherein said first and said second amino acid sequences are contiguous and in sequential order.

An isolated chimeric polypeptide, comprising a first amino acid sequence being at least about 90% homologous to amino acids 1-35 of known PYY (SEQ ID NO:66), and a second amino acid sequence being at least 70% homologous to a peptide having the sequence SEGPDLW (SEQ ID NO:112) corresponding to amino acids 3642 of PYY skipping exon 3 [1-42] variant (SEQ ID NO:74), wherein said first and said second amino acid sequences are contiguous and in sequential order.

A chimeric polypeptide comprising an edge portion of PYY skipping exon 3 variant (SEQ ID NO:74), having a length “n”, wherein n is at least about 10 amino acids, comprising the dipeptide RS, the edge portion having a sequence starting from amino acid number 35−x (numbering according to SEQ ID NO:74) and extending to amino acid number 36+((n−2)−x), in which x is an integer from 0 to n−2, wherein the value ((n−2)−x) is not larger than 6.

A pharmaceutical composition comprising as an active ingredient a PYY Splice Variant polypeptide having an amino acid sequence as set forth in any one of SEQ ID NOS:70-75.

An isolated nucleic acid molecule encoding a PYY variant having a nucleic acid sequence as set forth in any one of SEQ ID NOS:80-83 or homologs thereof.

A pharmaceutical composition comprising as an active ingredient a nucleic acid molecule encoding a PYY variant having a nucleic acid sequence as set forth in any one of SEQ ID NOS:80-83 or homologs thereof.