Adiponectin fragments and conjugates
AssigneeMaxygen Aps Maxygen Holdings, Ltd.
InventorsPoul Rasmussen, Kim Andersen, Anders Pedersen, Hans Schambye, Torben Halkier, Are Bogsnes
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Abstract
The invention relates to a conjugate comprising an adiponectin polypeptide, and a first non-polypeptide moiety covalently attached to the adiponectin polypeptide, wherein the adiponectin polypeptide comprises an amino acid residue having an attachment group for said first non-polypeptide moiety, wherein said amino acid residue has been introduced in a position that in the parent adiponectin is occupied by a surface exposed amino acid residue.
Claims21
1-76. (canceled)
An adiponectin polypeptide consisting essentially of a globular domain and a collagen domain, wherein
- the globular domain comprises (a) an amino acid sequence starting at position 108 and ending at position 244 of SEQ ID NO:1 or (b) a sequence that differs from the amino acid sequence of (a) in one to eleven substitution(s);
- and
- the collagen domain comprises an amino acid sequence starting at between positions 42 and 100 inclusive and ending at position 107 of SEQ ID NO:1, and comprises at least one lysine which is hydroxylated and glycosylated.
A method for producing an adiponectin polypeptide comprising a non-polypeptide moiety, the method comprising
- (i) providing an adiponectin polypeptide consisting essentially of a globular domain and a collagen domain, wherein:
- the globular domain comprises one to eleven substitution(s) relative to the amino acid sequence starting at position 108 and ending at position 244 of SEQ ID NO:1, wherein said one to eleven substitution(s) comprise at least one introduced amino acid residue in a position which in human adiponectin (SEQ ID NO:1) is occupied by a surface-exposed amino acid residue, the introduced amino acid residue comprising an attachment group for a first non-polypeptide moiety, and
- the collagen domain comprises an amino acid sequence starting at between positions 42 and 100 inclusive and ending at position 107 of SEQ ID NO:1;
- and
- (ii) covalently attaching the first non-polypeptide moiety to the attachment group of the introduced amino acid.
Dependencies are inferred from the claim text.