Size-exclusion ion-exchange particles
AssigneeApplera Corporation
InventorsAldrich Lau, Michael Harrold
Life of this patent
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Abstract
A size-exclusion ion-exchange particle and a device using the particle are provided wherein the particle includes an ion-exchange core micro-encapsulated by a shell, and the shell includes a polymerization product of a reactive monomer. The shell can be an at least partially cross-linked polymer. The shell can be capable of excluding molecules of a size equal to or larger than a 10 nt ssDNA molecule or molecules of a size equal to or larger than a 100 nt ssDNA molecule, for example, and the core can be capable of ion-exchange. Methods of making size-exclusion ion-exchange particles are also provided, as are methods of purification using the particles.
Claims22
-50. (canceled)
A method of purifying a sample, comprising:
- providing a plurality of size-exclusion ion-exchange particles, wherein each particle comprises a core for ion-exchange and a shell for size-exclusion;
- contacting the sample with the particles to form a purified sample;
- and
- separating the purified sample from the particles.
-60. (canceled)
A method of purifying a biological sample, comprising:
- providing a plurality of particles, wherein at least some of the particles comprise a ion-exchange portion and a size-exclusion portion;
- contacting the biological sample with the particles to form a purified sample, wherein the purified sample comprises:
- (a) nucleic acid having a molecular size of 10 nt ssDNA or greater;
- and
- (b) salt having a concentration less than or equal to 50 μM;
- and
- separating the nucleic acid from the particles.
A method of purifying a biological sample, comprising:
- providing the biological sample, wherein the biological sample comprises DNA sequencing reaction products;
- providing a plurality of particles, wherein at least some of the particles comprise an ion-exchange property and a size-exclusion property;
- contacting the biological sample with the particles to form a purified sample;
- separating the purified sample from the particles;
- and
- sequencing the purified sample, wherein the sequencing does not include blobs.
Dependencies are inferred from the claim text.