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US 2006/0051348 A1Published application
Method of producing a plurality of isolated antibodies to a plurality of cognate antigens
OwnerJorn Gorlachinventor
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Abstract
The present invention relates to a method for producing high affinity antibodies that are antigen-specific. The method involves binding a plurality of antibody-producing B-cells from a mammal to a plurality of cognate antigens; sorting the bound antibody-producing B-cell and cognate antigen; amplifying nucleic acid sequences encoding each antibody, or fragment thereof, from the B-cells; and expressing the each antibody in a protein expression system. Antibodies produced in this manner are useful in diagnostic and therapeutic applications.
Claims2
A method for producing a plurality of isolated antibodies to a plurality of cognate antigens comprising
- binding a plurality of antibody-producing B-cells from a mammal to a plurality of cognate antigens;
- isolating each bound antibody-producing B-cell and cognate antigen;
- amplifying nucleic acid sequences encoding each antibody, or fragment thereof, from the B-cells;
- introducing each nucleic acid sequence encoding each antibody, or fragment thereof, into an expression system capable of expressing an antibody so that a plurality of isolated antibodies to a plurality of cognate antigens is produced.
1 more element
Dependencies are inferred from the claim text.