Optimization of transgene expression in mammalian cells
OwnerJacques Mallet, Sophie Brun +2inventors
InventorsJacques Mallet, Sophie Brun, Noelle Dufour, Nicole Faucon-Biguet
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Abstract
The present invention relates to vectors, compositions and methods for delivering transgenes into mammalian cells. The invention also relates to genetic constructs and recombinant cells suitable to produce such transgenes. The invention more particularly relates to a vector suitable for transgene delivery into mammalian cells, wherein said vector comprises a chimeric genetic construct comprising a transgene operably linked to at least two distinct posttranscriptional regulatory elements functional in mammalian cells. This invention can be used in experimental, research, therapeutic, prophylactic or diagnostic areas.
Claims34
1-34. (canceled)
A vector suitable for transgene delivery into mammalian cells, wherein said vector comprises a chimeric genetic construct comprising a transgene operably linked to at least two distinct posttranscriptional regulatory elements functional in mammalian cells.
A vector suitable for transgene delivery into mammalian cells, wherein said vector comprises a chimeric genetic construct comprising a transgene operably linked to a WPRE element and to an APP5′UTR region.
A vector suitable for transgene delivery into mammalian cells, wherein said vector comprises a chimeric genetic construct comprising a transgene operably linked to a WPRE element, an APP5′UTR region and a tau3′UTR region.
A vector suitable for transgene delivery into mammalian cells, wherein said vector comprises a chimeric genetic construct comprising a transgene operably linked to a WPRE element, an APP5′UTR region, a tau3′UTR region and a TH3′UTR region.
A method of expressing a transgene in a mammalian cell in vitro or ex vivo, the method comprising:
- a. providing a chimeric genetic construct comprising said transgene operably linked to at least two distinct posttranscriptional regulatory elements, and
- b. introducing said construct into mammalian cells, said introduction causing expression of said transgene in said mammalian cells.
A method of expressing a transgene in glial cells, the method comprising:
- e. providing a chimeric genetic construct comprising said transgene operably linked to posttranscriptional regulatory elements comprising a WPRE element combined with a APP5′UTR or a portion thereof, and
- f. introducing said construct into glial cells, said introduction causing expression of said transgene in said glial cells.
A method of expressing a transgene in fibroblasts, the method comprising:
- g. providing a chimeric genetic construct comprising said transgene operably linked to posttranscriptional regulatory elements comprising a WPRE element combined with a APP5′UTR or a portion thereof, and
- h. introducing said construct into fibroblasts, said introduction causing expression of said transgene in said fibroblasts.
A method of expressing a transgene in neuronal cells, the method comprising:
- i. providing a chimeric genetic construct comprising said transgene operably linked to posttranscriptional regulatory elements comprising a WPRE element combined with a APP5′UTR and a tau3′UTR or a portion thereof, and
- j. introducing said construct into neuronal cells, said introduction causing expression of said transgene in said neuronal cells.
A method of expressing a transgene in neuronal cells, the method comprising:
- k. providing a chimeric genetic construct comprising said transgene operably linked to posttranscriptional regulatory elements comprising a WPRE element combined with a APP5′UTR, a tau3′UTR and a TH3′UTR or a portion thereof,
- l. introducing said construct into neuronal cells, said introduction causing expression of said transgene in said neuronal cells.
Dependencies are inferred from the claim text.