Novel self-assembly molecules
AssigneeNational Research Council of Canada
InventorsC Mackenzie, Jianbing Zhang
Life of this patent
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Abstract
There is provided a method of forming a multimeric complex having affinity for a target. The method comprises: obtaining a plurality of self-assembly molecules, said self-assembly molecules including complementary self-assembly units such as verotoxin subunit B, each of which is operatively connected to an interaction domain such as a single domain antibody specific for the target; and combining said self-assembly molecules such that at least three said self-assembly units simultaneously bind to one another so as to permit the single domain antibodies to bind the target.
Claims44
A method of forming a multimeric complex having affinity for a target, said method comprising:
- obtaining a plurality of AB5 derived self-assembly molecules, said self-assembly molecules including complementary self-assembly units each of which is operatively connected to an interaction domain specific for the target;
- combining said self-assembly molecules such that at least three said self-assembly units substantially simultaneously bind to one another and permit the interaction domain to bind the target.
A self-assembly molecule having an affinity for one or more known targets and suitable for use in the formation of a multimeric complex, comprising:
- a AB5-derived self-assembly unit;
- and,
- an interaction domain operatively connected to said self-assembly unit so as to permit binding of said interaction domain to the target and to permit binding of said self-assembly unit to another self-assembly unit.
A self-assembly molecule comprising a plurality of proteinaceous or peptide portions operatively connected together, a first said portion comprising an AB5 derived self-assembly region capable of binding to the self-assembly regions of at least two other self-assembly molecules of the same or different composition, to form a complex of at least three self-assembly molecules, and a second said portion comprising an interaction domain adapted to specifically interact with a target region on another, different molecule.
A method of diagnosing the presence of a plurality of molecules on a surface, said method comprising:
- obtaining a plurality of AB5 derived self-assembly molecules, each self-assembly molecule including a complementary self-assembly unit, an interaction domain specific for the molecule and connected to the self-assembly unit, and a marker;
- exposing said surface to the self-assembly molecules;
- permitting said subunits to self-assemble into a multimeric complex;
- and,
- assaying for the marker.
In a mammalian patient, a method of alleviating or reducing the symptoms of a condition characterized by excessive levels of a cell type, virus or substance having multiple substantially identical targets thereon, said method comprising:
- obtaining AB5 derived self-assembly molecules, each said self-assembly molecule including a complementary self-assembly unit operatively connected to both an interaction domain specific for said target, and a destructive material;
- administering an effective amount of the self-assembly molecules to the patient;
- and
- allowing the self-assembly molecules to recognize said target and form a multimeric complex, such that the destructive material causes the death, reducing or elimination of the cell type, virus or substance.
Dependencies are inferred from the claim text.