Device for staining and hybridization reactions
AssigneeIntavis Bioanalytical Instruments AG
InventorHeinrich Gausepohl
Life of this patent
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Abstract
A device to perform complex protocols in immuno-cytochemistry and in situ hybridization on specimens attached to microscope sliders wherein such device encloses a biological material attached to a glass slide in such a way that a capillary gap is formed in combination with a cover plate equipped with spacers and allows individual treatment of each slide with reagents while also allowing automated liquid exchange with no cross contamination with the use of low reagent volume and the controlled evaporation during long incubations and elevated temperature as required for in situ hybridization in a simple and compact design for easy handling.
Claims22
Assembly of construction elements comprising two or more sets of construction elements, wherein each set comprises
- a slide (1),
- a cover plate (3),
- one ore more spacer elements (4) adapted to keep the slide (1) and the cover plate (3) parallel to each other at a distance corresponding to a capillary gap (9),
- a sealing element (5) for separating each pair of slide (1) and cover plate (3) from a neighbouring pair and forming a reservoir above the capillary gap in cooperation with the neighbouring sealing element and
- a compression device for compressing the sets together and holding the slides (1) with their cover plates (3) in an essentially vertical position.
Procedure for treatment of biological samples attached to a slide (1) in which a cover plate (3) is posed across the slide (1) with a capillary gap (9) formed between them and which is held in a vertical position and in which liquids are delivered on the top edge of the gap (9) and exit at the bottom.
Dependencies are inferred from the claim text.