US 2006/0051242 A1Published application

Measurement and calibration method for sample injection volume and mobile phase delivery rate in a ultra micro-scale liquid phase delivery system

AssigneeAtomic Energy Council-Institute of Nuclear Energy Research

InventorsKung-Tien Liu, Jyh-Perng Chiu, Hsuan-Erh Chao, Ying-Kai Fu

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Abstract

The present invention provides a method for measuring and calibrating sample injection volume or mobile phase delivery rate of any type of micropumps or their integrated systems by using the direct proportion of the total activity (or mass) to the delivery rate (or volume) of a radiochemical nuclide with known activity in a certain time period. Also, the present invention may adjust the range of measurement and calibration from micro-liter (μL) to pico-liter (pL) or from μL/min to pL/min by selecting nuclide species and their concentrations from different liquid calibration radiation sources.

Claims20

Dependent claims

A method for measuring the sample injection volume and the mobile phase delivery rate in an ultra micro-scale liquid phase delivery system, comprising the following steps:

(1) selecting a suitable radiochemical substance as a nuclide of the sample to prepare a source solution, based on the micro grade of the volume and the flow rate to be measured;
(2) collecting the source solution for a time period t and measuring the counting rate of the collected source solution by a radioehemieal activity counting apparatus;
and
(3) calculating the volume and the delivery rate of the collected source solution by Formula A and Formula B;
V
total
=
R
n
-
R
B
C
n
×
M
n
×
Sp
n
×
Eff
n
Formula
⁢
⁢
A
f
m
=
R
n
-
R
B
C
n
×
M
n
×
Sp
n
×
Eff
n
×
t
Formula
⁢
⁢
B
wherein, Vtotal is the sample volume, fm is the delivery rate, Rn is the counting rate of the source solution, RB is the background counting rate, Cn is the concentration of the source solution, Mn is the atomic mass of the nuclide, Spn is the specific activity of the nuclide, Effn is the counting efficiency of the nuclide, and t is the collection time.

50 more elements

Dependencies are inferred from the claim text.

Facts

Application: 11/119,215Filed 29 Apr 2005
Priority: TW 0931273199 Sep 2004
Published: 9 Mar 2006
Term ends: Not estimatedThe source records don’t support an estimate.

Classification8 codes

G01F 25/0092
Testing or calibration of apparatus for measuring volume, volume flow or liquid level or for metering by volume: for metering by volume
G01F 1/7042
Using marked regions or existing inhomogeneities within the fluid stream, e.g. statistically occurring variations in a fluid parameter: using…
G01N 35/1016
Characterised by arrangements for controlling the aspiration or dispense of liquids: control of the volume dispensed or introduced
G01N 2035/1034
General features of the devices: transferring microquantities of liquid