Nicotiana nucleic acid molecules and uses thereof
AssigneeU.S. Smokeless Tobacco Company LLC
InventorsDongmei Xu, Mark Nielsen
Life of this patent
Estimating the term…
Abstract
The present invention features Nicotiana nucleic acid sequences such as sequences encoding constitutive, or ethylene or senescence induced polypeptides, in particular cytochrome p450 enzymes, in Nicotiana plants and methods for using these nucleic acid sequences and plants to alter desirable traits, for example by using breeding protocols.
Claims94
A breeding method for producing a tobacco plant having decreased expression of a nicotine demethylase gene, said method comprising the steps of:
- (a) providing a first tobacco plant having variant nicotine demethylase gene expression;
- (b) providing a second tobacco plant that contains at least one phenotypic trait;
- (c) crossing said first tobacco plant with said second tobacco plant to produce an F1 progeny plant;
- (d) collecting seed of said F1 progeny for said variant nicotine demethylase gene expression;
- and
- (e) germinating said seed to produce a tobacco plant having decreased expression of a nicotine demethylase gene.
A method of breeding a nicotine demethylase deficiency trait into a tobacco plant, said method comprising the steps of:
- a) crossing a first tobacco plant having variant nicotine demethylase gene expression with a second tobacco plant;
- b) producing progeny tobacco plants of the cross;
- c) extracting a DNA sample from progeny tobacco plants;
- d) contacting the DNA sample with a marker nucleic acid molecule that hybridizes to a nicotine demethylase gene or fragment thereof;
- and
- e) performing a marker assisted breeding method for the variant nicotine demethylase gene expression trait.
A method of producing tobacco seed, comprising crossing any one of the tobacco plants selected from the group consisting of Nicotiana africana, Nicotiana amplexicaulis, Nicotiana arentsii, Nicotiana benthamiana, Nicotiana bigelovii, Nicotiana corymbosa, Nicotiana debneyi, Nicotiana excelsior, Nicotiana exigua, Nicotiana glutinosa, Nicotiana goodspeedii, Nicotiana gossei, Nicotiana hesperis, Nicotiana ingulba, Nicotiana knightiana, Nicotiana maritima, Nicotiana megalosiphon, Nicotiana miersii, Nicotiana nesophila, Nicotiana noctiflora, Nicotiana nudicaulis, Nicotiana otophora, Nicotiana palmeri, Nicotiana paniculata, Nicotiana petunioides, Nicotiana plumbaginifolia, Nicotiana repanda, Nicotiana rosulata, Nicotiana rotundifolia, Nicotiana rustica, Nicotiana setchelli, Nicotiana stocktonii, Nicotiana eastii, Nicotiana suaveolens, and Nicotiana trigonophylla with itself or with a second, distinct tobacco.
A method for developing a tobacco plant in a tobacco breeding program comprising:
- (a) providing a tobacco plant, or its components, having variant nicotine demethylase gene expression;
- and
- (b) employing said plant or components as a source of breeding material using tobacco plant breeding techniques.
A breeding method for producing a tobacco plant having a modified attribute, said method comprising the steps of:
- (a) providing a first tobacco plant having a modified attribute comprising variant gene expression of a nucleic acid molecule selected from the group consisting of the nucleic acid sequences shown in FIG. 1, FIGS. 3 to 7, FIGS. 10 to 158, FIGS. 162 to 170, FIGS. 172-1 to 172-19, and FIGS. 173-1 to 173-294;
- (b) providing a second tobacco plant that contains at least one phenotypic trait;
- (c) crossing said first tobacco plant with said second tobacco plant to produce an F1 progeny plant;
- (d) collecting seed of said F1 progeny for said modified attribute;
- and
- (e) germinating said seed to produce a tobacco plant having said modified attribute.
A method of breeding an attribute into a tobacco plant, said method comprising the steps of:
- a) crossing a first tobacco plant having a modified attribute comprising variant gene expression of a nucleic acid molecule selected from the group consisting of the nucleic acid sequences shown in FIG. 1, FIGS. 3 to 7, FIGS. 10 to 158, FIGS. 162 to 170, FIGS. 172-1 to 172-19, and FIGS. 173-1 to 173-294 with a second tobacco plant;
- b) producing progeny tobacco plants of the cross;
- c) extracting a DNA sample from progeny tobacco plants;
- d) contacting the DNA sample with a marker nucleic acid molecule that hybridizes to a nucleic acid sequence shown in FIG. 1, FIGS. 3 to 7, FIGS. 10 to 158, FIGS. 162 to 170, FIGS. 172-1 to 172-19, and FIGS. 173-1 to 173-294 or a fragment thereof;
- and
- e) performing a marker assisted breeding method for the modified attribute.
A method of producing tobacco seed, comprising crossing a first tobacco plant selected from the group consisting of Nicotiana africana, Nicotiana amplexicaulis, Nicotiana arentsii, Nicotiana benthamiana, Nicotiana bigelovii, Nicotiana corymbosa, Nicotiana debneyi, Nicotiana excelsior, Nicotiana exigua, Nicotiana glutinosa, Nicotiana goodspeedii, Nicotiana gossei, Nicotiana hesperis, Nicotiana ingulba, Nicotiana knightiana, Nicotiana maritima, Nicotiana megalosiphon, Nicotiana miersii, Nicotiana nesophila, Nicotiana noctiflora, Nicotiana nudicaulis, Nicotiana otophora, Nicotiana palmeri, Nicotiana paniculata, Nicotiana petunioides, Nicotiana plumbaginifolia, Nicotiana repanda, Nicotiana rosulata, Nicotiana rotundifolia, Nicotiana rustica, Nicotiana setchelli, Nicotiana stocktonii, Nicotiana eastii, and Nicotiana suaveolens with a second tobacco plant having a modified attribute comprising variant gene expression of a nucleic acid molecule selected from the group consisting of the nucleic acid sequences shown in FIG. 1, FIGS. 3 to 7, FIGS. 10 to 158, FIGS. 162 to 170, FIGS. 172-1 to 172-19, and FIGS. 173-1 to 173-294.
A method for developing a tobacco plant in a tobacco breeding program comprising:
- (a) providing a tobacco plant, or its components, having a modified attribute comprising variant gene expression of a nucleic acid molecule selected from the group consisting of the nucleic acid sequences shown in FIG. 1, FIGS. 3 to 7, FIGS. 10 to 158, FIGS. 162 to 170, FIGS. 172-1 to 172-19, and FIGS. 173-1 to 173-294;
- and
- (b) employing said plant or components as a source of breeding material using tobacco plant breeding techniques.
An isolated genetic marker comprising a nucleic acid sequence that is substantially identical to a nucleic acid sequence selected from the group consisting of the nucleic acid sequences shown in FIG. 1, FIGS. 3 to 7, FIGS. 10 to 158, FIGS. 162 to 170, FIGS. 172-1 to 172-19, and FIGS. 173-1 to 173-294.
An expression vector comprising a nucleic acid sequence selected from the group consisting of the nucleic acid sequences shown in FIG. 1, FIGS. 3 to 7, FIGS. 10 to 158, FIGS. 162 to 170, FIGS. 172-1 to 172-19, and FIGS. 173-1 to 173-294, said vector being capable of directing expression of the polypeptide encoded by said nucleic acid sequence.
A plant or plant component comprising an isolated nucleic acid sequence selected from the group consisting of the nucleic acid sequences shown in FIG. 1, FIGS. 3 to 7, FIGS. 10 to 158, FIGS. 162 to 170, FIGS. 172-1 to 172-19, and FIGS. 173-1 to 173-294, wherein said nucleic acid sequence is expressed in said plant or said plant component.
A method for reducing the expression or enzymatic activity of a constitutive, or an ethylene induced or senescence induced tobacco polypeptide in a plant cell, said method comprising reducing the level or enzymatic activity of an endogenous constitutive, or ethylene or senescence induced tobacco polypeptide in said plant cell.
A method for increasing the expression or enzymatic activity of a constitutive, or an ethylene or senescence induced tobacco polypeptide in a plant cell, said method comprising increasing the level or enzymatic activity of an endogenous constitutive, or ethylene or senescence induced tobacco polypeptide in said plant cell.
Dependencies are inferred from the claim text.