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US 2006/0041387 A1Published application
Smart microarray cancer detection system
OwnerXiumei Suninventor
Life of this patent
Estimating the term…
Abstract
A smart microarray detection system for exploring and detecting cancer specific genes. A microarray is constructed using cancer specific genes that can detect, identify, quantify, and discriminate cancers in biological specimens. Data obtained from the microarray is analyzed with questionable signals reanalyzed with real time PCR. This information is then evaluated using software to determine a cancer prognosis.
Claims15
1) A method for diagnosing cancer in a subject, comprising the steps of:
- obtaining a tissue sample taken from a tumor suspected of being cancerous;
- obtaining a tissue sample taken from known normal tissue;
- labeling both tissue samples using biotin;
- performing real time PCR and preparing biotinylated cDNA probes for hybridization;
- hybridizating the biotinylated probes using standard protocols;
- analyzing the probes using a microarray;
- detecting the results using chemiluminescence;
- analyzing the date using a commercial software package;
- and generating a prognosis of cancer.
6 more elements
10) A system for diagnosing cancer in a subject, comprising:
- a first tissue sample taken from a tumor suspected of being cancerous;
- a second tissue sample taken from known normal tissue;
- means for labeling said first and second tissue samples using biotin;
- means for performing real time PCR and preparing biotinylated cDNA probes for hybridization;
- a hybridization oven for hybridizing the biotinylated probes;
- a tumor gene marker microarray chip for generating data relative to a cancer diagnosis;
- a CCD image analyzer for scanning said microarray chip to detect the generated data;
- computer means for analyzing the data generated using a commercial software package;
- means for performing real time PCR again to clarify any noisy signals and any weak and unclear signals;
- computer means for reanalyzing the signals and providing clinical diagnostic and prognostic evaluation;
- and readout means for displaying prognostic information concerning cancer.
8 more elements
Dependencies are inferred from the claim text.