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US 2006/0041384 A1Published application
Automated information processing in randomly ordered arrays
AssigneeIllumina, Inc.
InventorsBahram Kermani, Juergen Haas
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Abstract
The invention relates to the use of a computer system to compare images generated from a randomly ordered array. This system preserves the relative position of each site within the array so that the same site can be compared in different images.
Claims10
A method of registering an analytical image of a microsphere array comprising:
- a) providing a microsphere array, wherein said microsphere array comprises:
- i) a substrate with a surface comprising discrete sites;
- and
- ii) a population of microspheres comprising at least a first and a second subpopulation each comprising a bioactive agent;
- wherein said micropsheres are distributed on said surface such that each of said discrete sites contain no more than 1 microsphere;
- b) decoding said microsphere array so that the location of each bioactive agent is elucidated;
- c) computing a registration grid based on known locations of said bioactive agents from information obtained from step b;
- d) contacting said array with a sample comprising target analytes;
- e) acquiring a hybridization intensity image of said microsphere array, wherein said hybridization intensity image further comprises at least two bright bead types to serve as a fiducials;
- f) overlaying a registration grid to said hybridization intensity image;
- g) aligning the registration grid with the hybridization intensity image;
- and
- h) identifying the intensity at each grid location for each bead type within said array.
10 more elements
A method of registering an analytical image comprising:
- a) obtaining a decoded image of a microsphere array, wherein said microsphere array comprises:
- i) a substrate with a surface comprising discrete sites;
- and
- ii) a population of microspheres comprising at least a first and a second subpopulation each comprising a bioactive agent;
- wherein said micropsheres are distributed on said surface such that each of said discrete sites contain no more than 1 micropshere;
- b) acquiring a hybridization image;
- c) overlaying a registration grid to said hybridization intensity image;
- d) aligning the registration grid with the hybridization intensity image;
- and
- e) identifying the intensity at each grid location for each bead type within said array;
- f) sum the signal intensity from at least 5 array locations corresponding to the predicted position of bright bead types, whereby if the image is aligned correctly, the sum of said signal intensities is at least 10 times higher than if the image is misaligned.
8 more elements
Dependencies are inferred from the claim text.