Renilla reniformis green fluorescent protein
AssigneeRutgers, The State University of New Jersey
InventorsWilliam Ward, Catherine Thomson
Life of this patent
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Abstract
Green fluorescent protein (GFP) polypeptides from Renilla reniformis and Renilla kollikeri are disclosed. The amino acid sequence of R. reniformis GFP and back-translated nucleotide sequences of nucleic acids encoding the R. reniformis GFP are also disclosed. These isolated polypeptides, along with the pertinent amino acid and nucleotide sequence information, are useful in a variety of applications for which GFPs from other sources (e.g., Aequoria) are currently employed. Techniques for using the Renilla GFPs are disclosed, along with advantages of Renilla GFP as compared with currently available GFPs.
Claims7
1-11. (canceled)
An isolated or synthesized nucleic acid molecule which encodes a polypeptide having an amino acid sequence that confers upon the polypeptide physical and biochemical properties of a green fluorescent protein (GFP) from Renilla reniformis or Renilla kollikeri.
An isolated nucleic acid molecule which encodes an isolated GFP comprising an amino acid sequence substantially the same as a sequence selected from the group consisting of SEQ ID NO:1 SEQ ID NO:3 and SEQ ID NO:46.
17-31. (canceled)
Dependencies are inferred from the claim text.