Indirect detection of cardiac markers for assessing acute myocardial infarction

A method for detecting an AMI-indicating antigen in a physiological sample comprising:

• combining the physiological sample with magnetic microparticles linked to a first biotinylated antibody, said first antibody binding to a first site on the AMI-indicating antigen present in the sample;
• applying a magnetic field to the physiological sample to separate the linked myoglobin from the blood sample to form a test sample comprising the magnetic particle-linked AMI-indicating antigen;
• combining the test sample with a second biotinylated antibody, said second antibody binding to a second site on the AMI-indicating antigen, wherein said second antibody is linked to a glucose molecule;
• and
• detecting the glucose in the test sample to determine the concentration of AMI-indicating antigen in the blood sample.

An immunoassay system comprising:

• a first antibody linkable to an assayable protein antigen present in a physiological fluid;
• a magnetic microparticle linked to the first antibody;
• a second antibody linkable to the assayable protein antigen;
• and
• a glucose molecule linked to the second antibody.

A method for detecting a target antigen in a physiological fluid comprising:

• linking via a first antibody a magnetic microparticle to the target antigen in the physiological fluid;
• utilizing a magnetic field to separate the magnetic micro particle with the target antigen linked thereto from at least a portion of other components in the physiological fluid, thereby forming a test sample;
• linking via a second antibody an enzymatic tag or glucose molecule to the target antigen;
• and
• detecting, using a calorimetric method, the linked enzymatic tag or glucose molecule in the test sample to determine the concentration of target antigen in the physiological fluid.