Biosensor
AssigneeFuji Photo Film Co., Ltd.
InventorsTaisei Nishimi, Toshihide Ezoe, Toshiaki Kubo
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Abstract
It is an object of the present invention to provide a technique of converting carboxylic acid into an active ester with no generation of air bubbles and a technique of stabilizing the obtained active ester. The present invention provides a biosensor wherein a carboxyl group existing on the surface of a substrate thereof is activated with any one compound selected from an uronium salt, a phosphonium salt or a triazine derivative which are defined in the present application, so as to form a carboxylic acid amide group.
Claims20
A biosensor, wherein a carboxyl group existing on the surface of a substrate thereof is activated with any one compound selected from the group consisting of an uronium salt represented by the following formula A1, a phosphonium salt represented by the following formula A2, and a triazine derivative represented by the following formula A3, so as to form a carboxylic acid amide group:
- wherein, in the formula A1, each of R1 and R2 independently represents an alkyl group having 1 to 6 carbon atoms, or R1 and R2 together form an alkylene group having 2 to 6 carbon atoms, which forms a ring together with an N atom, R3 represents an aromatic group having 6 to 20 carbon atoms or a heterocyclic group containing at least one heteroatom, and X− represents an anion;
- in the formula A2, each of R4 and R5 independently represents an alkyl group having 1 to 6 carbon atoms, or R4 and R5 together form an alkylene group having 2 to 6 carbon atoms, which forms a ring together with an N atom, R6 represents an aromatic group having 6 to 20 carbon atoms or a heterocyclic group containing at least one heteroatom, and X− represents an anion;
- and in the formula A3, R7 represents an onium group, and each of R8 and R9 independently represents an electron-donating group.
A biosensor, wherein a carboxyl group existing on the surface of a substrate thereof is activated with a carbodiimide derivative or a salt thereof, and it is then converted into an ester using any one compound selected from the group consisting of a nitrogen-containing heteroaromatic compound having a hydroxyl group, a phenol derivative having an electron-withdrawing group, and an aromatic compound having a thiol group, followed by a reaction with, amine, so as to form a carboxylic acid amide group.
Dependencies are inferred from the claim text.