RNA interference vectors
AssigneePromega Corporation
InventorsCheryl Bailey, Doug Storts, Martin Rosenberg
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Abstract
The present invention relates to gene-specific silencing through RNA interference, and in particular, to vectors for expressing RNAi molecules. In some embodiments, the present invention provides compositions and methods for inducible expression of RNAi molecules, and/or for long-term expression of RNAi molecules. Hence the compositions and methods described herein are suitable for regulatable and/or sustained gene-specific silencing in cells.
Claims27
A composition comprising a vector, said vector comprising an snRNA pol II promotor operably associated with an RNAi molecule.
A kit for cloning an RNAi molecule, comprising:
- i) an snRNA RNA polymerase II promoter template oligonucleotide, ii) a primer complementary to said template, iii) a vector, iv) amplification reagents, and v) ligation reagents.
A kit comprising a vector, said vector comprising an snRNA pol II promotor operably associated with an RNAi molecule.
Dependencies are inferred from the claim text.