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US 2006/0040389 A1Published application
Purified compositions of stem cell derived differentiating cells
OwnerCharles Murry, Michael Laflammeinventors
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Abstract
Viable differentiating cells from in vitro cultures of stem cells are selected for by partial dissociation to provide cell aggregates. Aggregates comprising cells of interest are selected for phenotypic features using methods that substantially maintain the cell to cell contacts in the aggregate.
Claims20
A method of enriching for differentiating cells of interest, the method comprising:
- partially dissociating an in vitro culture comprising human stem cells grown under differentiative conditions, to provide a population of cell aggregates;
- selecting said cell aggregates for a phenotypic feature of interest present on said differentiating cells;
- wherein an enriched cell population comprising differentiating cells of interest is obtained.
Dependencies are inferred from the claim text.