Protein microarrays
AssigneeBiocept, Inc.
InventorPavel Tsinberg
Life of this patent
Estimating the term…
Abstract
Methods for making a microarray having minimal background binding of proteins by appropriately coating a substrate surface which is initially derivatized with organic functional groups. A protein-resistant polymeric coating is applied which has hydrophilic backbone polymers that are crosslinked to a substantial degree via polyfunctional isocyanate moieties. Three dimensional hydrogel microspots containing capture agents are affixed at distinct spatial locations across an array region of the surface to form a microarray. The microspots are affixed either to the substrate or to the coating. The polymeric coating preferably comprises isocyanate-capped PEG crosslinked with a polyfunctional isocyanate to form urethane polymers.
Claims20
A microarray which comprises:
- a substrate having a flat upper surface which is derivitized to carry organic functional groups,
- a plurality of three-dimensional (3D) microspots at discrete spatial locations across an array region of said surface, which microspots contain or are adapted to link directly or indirectly to an organic capture agent, and
- a protein-resistant polymeric coating covering the surface in the array region surrounding the microspots, which polymeric coating is multifunctional, comprising hydrophilic backbone polymers, which polymers are crosslinked to a substantial degree via polyfunctional isocyanate molecules, said multifunctional coating being covalently bound to said organic functional groups on said surface via isocyanate linking and providing free isocyanate groups.
A method for making a microarray that minimizes background binding, which method comprises:
- providing a substrate having a flat upper surface which is derivatized with organic functional groups,
- applying a protein-resistant polymeric coating to cover at least an assay region of said surface by covalently binding said coating to said organic functional groups, which polymeric coating is multifunctional comprising hydrophilic backbone polymers which backbone polymers are cross-linked to a substantial degree via polyfunctional isocyanate molecules,
- curing said coating,
- affixing a plurality of three-dimensional hydrogel spots at discrete spatial locations across within said array region of said surface, and
- linking different organic capture agents of interest into various of said three-dimensional spots.
Dependencies are inferred from the claim text.