Recombinant human Mannan-Building Proteins
AssigneeFuso Pharmaceutical Industries, Ltd.
InventorNobutaka Wakamiya
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Abstract
Recombinant Human Mannan-Binding Proteins (rhMBP) having physiological activities which are substantially identical to those offered by Human Mannan-Binding Proteins (hMBP), as well as, in particular, a production system for homogenously producing rhMBP having the specific peaks at the molecular weight of 1,000˜1,300 kDa determined by absorbance (280 nm) in Gel-Filtration Chromatography are provided.
Claims7
1-17. (canceled)
Recombinant Human Mannan-Binding Proteins (rhMBP) produced by a Chinese Hamster Ovary (CHO) cell which:
- lacks dihydrofolate reductase (dhfr−),
- possesses resistance to both neomycin and methotrexate, and is transformed with an expression vector pNOW1-hMBP comprising the plasmid pNOW1 and cDNA consisting of the nucleotide sequence of SEQ ID NO. 2;
- wherein the cDNA encodes the claimed rhMBP.
The rhMBP according to claim 1 wherein said cDNA is the continuous nucleotide sequence of from 66th nucleotide to 812th nucleotide in the nucleotide sequence set out in SEQ ID NO:
- 1.
The rhMBP according to claim 2 wherein said continuous nucleotide sequence encodes the continuous amino acid sequence of from 21st amino acid to 248th amino acid in the amino acid sequence set out in SEQ ID NO:
- 28.
The rhMBP according to claim 3 which has a specific peak at molecular weight of 1,000 kDa-1,300 kDa as determined by 280 nm absorbance in Gel-Filtration Chromatography.
The rhMBP according to claim 3 which has a specific peak at molecular weights of 200 kDa-400 kDa as determined by 280 nm absorbance in Gel-Filtration Chromatography.
The rhMBP according to claim 3 which has specific peaks at molecular weight of 1,000 kDa-1,300 kDa and 200 kDa-400 kDa as determined by 280 nm absorbance in Gel-Filtration Chromatography.
Dependencies are inferred from the claim text.