Mutated Tn5 transposase proteins and the use thereof
AssigneeWisconsin Alumni Research Foundation
InventorsWilliam Reznikoff, Richard Gradman
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Abstract
Transposase proteins that are modified relative to and have higher transposase activities than the wild-type Tn5 transposase are disclosed. A transposase protein of the present invention differs from the wild-type Tn5 transposase at amino acid position 41, 42, 450, or 454 and has greater avidity than the wild-type Tn5 transposase for at least one of a Tn5 outside end sequence as defined by SEQ ID NO:3, a Tn5 inside end sequence as defined by SEQ ID NO:4, and a modified Tn5 outside end sequence as defined by SEQ ID NO:5. Also disclosed are various systems and methods of using the transposase proteins of the present invention for in vitro or in vivo transposition.
Claims20
A polypeptide comprising an amino acid sequence of a wild-type Tn5 transposase modified at an amino acid position selected from the group consisting of position 41, position 42, position 450, and position 454, wherein a wild-type Tn5 transposase with said modification has greater avidity than an unmodified wild-type Tn5 transposase for at least one of a Tn5 outside end sequence as defined by SEQ ID NO:3, a Tn5 inside end sequence as defined by SEQ ID NO:4, and a modified Tn5 outside end sequence as defined by SEQ ID NO:5.
Dependencies are inferred from the claim text.