Expression of mammalian proteins in Pseudomonas fluorescens
AssigneePfenex Inc.
InventorsDiane Retallack, Charles Squires, David Watkins, Stacey Lee, Frank Gaertner, Robert Shutter
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Abstract
The invention is a process for improved production of a recombinant mammalian protein by expression in a Pseudomonad, particularly in a Pseudomonas fluorescens organism. The process improves production of mammalian proteins, particularly human or human-derived proteins, over known expression systems such as E. coli in comparable circumstances Processes for improved production of isolated mammalian, particularly human, proteins are provided.
Claims30
1) A process for increasing expression of a recombinant mammalian protein comprising:
- a. transforming a Pseudomonas fluorescens host cell with a nucleic acid encoding a recombinant mammalian protein;
- and
- b. growing the cell under conditions that allow expression of the recombinant mammalian protein;
- wherein the protein is expressed at an increased level when compared to an expression level of the protein under substantially comparable conditions in an E. coli expression system.
13) A process for producing a recombinant mammalian protein in a Pseudomonas fluorescens host cell comprising:
- a. transforming a host cell with a nucleic acid encoding a recombinant mammalian protein;
- b. growing the cell under conditions that allow expression of the recombinant mammalian protein;
- and
- c. isolating the recombinant mammalian protein.
26) A process for producing a recombinant human protein in a host cell comprising:
- a. transforming a host cell with a nucleic acid encoding a recombinant human peptide;
- and
- b. growing the cell under conditions that allow expression of the recombinant human peptide;
- wherein the host cell is Pseudomonas fluorescens.
29) A Pseudomonas fluorescens cell comprising a nucleic acid encoding a recombinant human peptide.
Dependencies are inferred from the claim text.