Use of triplex structure DNA in transferring nucleotide sequences
OwnerPierre Charneau, Veronique Zennou +1inventors
InventorsPierre Charneau, Veronique Zennou, Huseyin Firat
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Abstract
The invention concerns a recombinant vector characterised in that it comprises a polynucleotide comprising a central initiation cis-active region (cPPT) and a termination cis-active region (CTS), said regions being of retroviral or retroviral-like origin, said vector further comprising a predetermined nucleotide sequence (transgene or nucleotide sequence of interest) and retranscription regulating, expressing and packaging signals of retroviral or retroviral-like origin.
Claims22
1-40. (canceled)
A recombinant vector, comprising:
- a polynucleotide comprising a cis-acting central initiation region (cPPT) and a cis-acting termination region (CTS), wherein the cPPT and CTS are of retroviral-like origin and derived from a retrotransposon;
- a defined nucleotide sequence (transgene or sequence of interest);
- and regulatory signals for reverse transcription, expression, and packaging, wherein said regulatory signals are of retroviral or retroviral-like origin.
A recombinant vector according to claim 48, wherein the HIV retrovirus is HIV-1 or HIV-2.
A polynucleotide comprising a nucleotide sequence of about 98 nucleotides, wherein said nucleotide sequence is from an HIV genome, wherein said nucleotide sequence is flanked on one side by a cis-acting central initiation nucleotide sequence (cPPT) comprising at least 10 nucleotides and on the other side by a cis-acting central termination nucleotide sequence (CTS) comprising at least 15 nucleotides, and wherein said polynucleotide comprises a segment of single-stranded or double-stranded polynucleotides.
A recombinant retroviral vector particle, comprising:
- (a) a gag polypeptide corresponding to a nucleoprotein of a lentivirus, or to a functional polypeptide derivative (GAG polypeptide);
- (b) a pol polypeptide constituted by the RT, PRO, and IN proteins of a lentivirus, or a functional polypeptide derivative (POL polypeptide);
- (c) an envelope polypeptide or a functional polypeptide derivative (ENV polypeptide);
- and
- (d) a recombinant nucleotide sequence, comprising:
- a defined nucleotide sequence (transgene or a sequence of interest), placed under the control of regulatory signals for transcription and expression;
- a sequence containing regulatory signals for reverse transcription, expression, and packaging, wherein the regulatory signals are of lentiviral origin;
- and a polynucleotide comprising a cis-acting central initiation region (cPPT) and a cis-acting termination region (CTS) of lentiviral origin, inserted in a functional orientation with said regulatory signals of retroviral or retroviral-like origin.
Dependencies are inferred from the claim text.