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US 2006/0040344 A1Published application
Microbial process for the preparation of 7-keto dehydroepiandrosterone and related analogs
OwnerMichael White, Doris Beck +1inventors
InventorsMichael White, Doris Beck, Ivan Gilbert
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Abstract
The present invention relates to a two part microbial process for the preparation of 7-oxo-5-androstene steroids of Formula III.
Claims7
A process for preparing 7-hydroxy-5-androstene steroids comprising the steps of:
- 1) contacting a 5-androstene steroid of Formula I
- wherein:
- R is H or —COR′;
- R′ is alkyl of 1 to 5 carbons;
- with a species of Mucor capable of performing a microbial transformation in a liquid culture at a steroid concentration of 1 gram per liter or greater to give a 7α-hydroxy-5-androstene steroid of Formula II;
- 2) isolating the 7α-hydroxy-5-androstene steroid of Formula II from the liquid culture of step 1).
3 more elements
A process for preparing 7-oxo-5-androstene steroids comprising the steps of:
- 1) contacting a 5-androstene steroid of Formula I
- wherein:
- R is H or —COR′;
- R′ is alkyl of 1 to 5 carbons;
- with a species of Mucor capable of performing a microbial transformation in a liquid culture to give a 7α-hydroxy-5-androstene steroid of Formula II;
- 2) isolating the 7α-hydroxy-5-androstene steroid of Formula II from the liquid culture of step 1);
- 3) contacting a 7-hydroxy-5-androstene steroid of Formula II in a liquid culture with an Escherichia species capable of oxidizing 7α-hydroxy-5-androstene steroids to a 7-oxo-5-androstene steroid of Formula III;
- 4) isolating the steroid of Formula III from the liquid culture of step 3.
5 more elements
Dependencies are inferred from the claim text.