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AssigneeBattelle Memorial Institute
InventorsZiyu Dai, Linda Lasure, Jon Magnuson
Estimating the term…
The present invention encompasses isolated gene regulatory elements and gene transcription terminators that are differentially expressed in a native fungus exhibiting a first morphology relative to the native fungus exhibiting a second morphology. The invention also encompasses a method of utilizing a fungus for protein or chemical production. A transformed fungus is produced by transforming a fungus with a recombinant polynucleotide molecule. The recombinant polynucleotide molecule contains an isolated polynucleotide sequence linked operably to another molecule comprising a coding region of a gene of interest. The gene regulatory element and gene transcription terminator may temporally and spatially regulate expression of particular genes for optimum production of compounds of interest in a transgenic fungus.
An isolated polynucleotide molecule comprising a polynucleotide sequence that regulates expression of a gene, wherein said gene is differentially expressed in a native fungus exhibiting a pelleted morphology relative to said native fungus exhibiting a filamented morphology.
An isolated polynucleotide molecule comprising a polynucleotide transcription terminator sequence from a gene that is differentially expressed in a native fungus exhibiting a pelleted morphology relative to said native fungus exhibiting a filamented morphology.
An isolated polynucleotide molecule comprising a promoter functional in fungi, wherein said promoter comprises at least a portion of a sequence in any one of SEQ ID NOs.:
An isolated polynucleotide molecule comprising a promoter having greater than 66.9% identity with the SEQ ID 50.
An isolated polynucleotide molecule comprising a transcription terminator, wherein said transcription terminator is contained in at least a portion of a sequence in any one of SEQ ID NOs.:
A DNA construct comprising the following elements operably linked in the direction of transcription:
Dependencies are inferred from the claim text.