Integrated cartridge for sample manipulation
AssigneeBecton, Dickinson and Company
InventorsBradley Thomas, Alan Harper, Charles Clemens, Raymond Clark, Robert Elms, Lanny Gorton, John Slate, Richard Meyst
Life of this patent
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Abstract
An integrated cartridge for automated sample manipulation and, particularly strand displacement amplification, is provided. The cartridge comprises a sealed, two-part device with internal fluid channels and chambers, as well as reagents. The cartridge performs the sequence of fluid transfers, reagent additions and heat transitions, such as those of the strand displacement amplification process, in a single, sealed device.
Claims17
A device for automated sample manipulation, comprising:
- a liquid inlet well for providing a test sample;
- a liquid input chamber for receiving said test sample from said liquid inlet well;
- a first reaction chamber for performing a first reaction on said test sample;
- a second reaction chamber for performing a second reaction on said test sample;
- a first heating surface for heating said first and second reaction chambers;
- and a denature chamber for denaturing said test sample.
A device for automated DNA amplification, comprising:
- a liquid inlet well for providing a test sample containing DNA;
- a liquid input chamber for receiving said test sample from said liquid inlet well;
- a first reaction chamber for performing a prime reaction on the DNA in said test sample;
- a second reaction chamber for performing an amplification reaction on the DNA in said test sample;
- a first heating surface for heating said first and second reaction chambers;
- and a denature chamber for denaturing the DNA in said test sample.
A device for automated sample manipulation, comprising a sealed cartridge having a cartridge top and a cartridge bottom, wherein said cartridge top comprises:
- a liquid inlet well;
- fluidic cavities;
- first, second and third connection ports;
- first and second heat block surfaces;
- and a take-out well.
-27. (canceled)
Dependencies are inferred from the claim text.