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US 2006/0040310 A1Published application
Epitope testing using soluble HLA
OwnerWilliam Hildebrand, Rico Buchli +2inventors
InventorsWilliam Hildebrand, Rico Buchli, Kiley Prilliman, Heather Hickman
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Abstract
The present invention relates generally to a methodology for assaying the binding of a peptide to an individual, specific, soluble HLA molecule. The peptides utilized in the method may be identified by indirect methods utilizing T lymphocytes, or by a direct method of epitope discovery described herein.
Claims24
A method of assaying a peptide for binding to an individual class I molecule, the method comprising the steps of:
- providing a peptide of interest;
- providing individual soluble class I molecule-endogenous peptide complexes in which individual soluble class I molecules have endogenous peptides loaded therein;
- mixing the peptide of interest with the individual soluble class I molecules;
- and
- identifying individual soluble class I molecule-peptide of interest complexes, wherein the individual soluble class I molecules have the peptide of interest loaded therein.
2 more elements
A method of assaying a peptide for binding to an individual class I molecule, the method comprising the steps of:
- providing a peptide of interest;
- labeling the peptide of interest;
- providing individual soluble class I molecules;
- mixing the labeled peptide of interest with the individual soluble class I molecules;
- and
- identifying individual soluble class I molecule-labeled peptide of interest complexes, wherein the individual soluble class I molecules have the labeled peptide of interest loaded therein.
3 more elements
Dependencies are inferred from the claim text.