Cleavage of nucleic acids
OwnerJames Prudent, Jeff Hall +3inventors
InventorsJames Prudent, Jeff Hall, Victor Lyamichev, Mary Brow, James Dahlberg
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Abstract
The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
Claims36
1-25. (canceled)
A method for detecting a target nucleic acid comprising exposing a composition comprising a purified thermostable FEN-1 endonuclease and a polymerase to a sample suspected of containing a target nucleic under conditions such that said target nucleic acid, if present in said sample, is detected.
Dependencies are inferred from the claim text.