Methods, compositions, and kits comprising PNA for RNA interference
AssigneeApplera Corporation
InventorGerald Zon
Life of this patent
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Abstract
The present teachings relate to methods, compositions, and kits for reducing the amount of a target polynucleotide sequence. In some embodiments, block oligomers comprising peptide nucleic acids (PNAs) are hybridized to an anti-sense strand to form an anti-sense complex comprising regions with desirable thermodynamic characteristics. In some embodiments, the expression of a target messenger RNA is reduced in RNA interference (RNAi) experiments using anti-sense compositions comprising PNAs.
Claims40
A method of reducing the amount of a target polynucleotide sequence comprising,
- providing,
- a. an anti-sense strand, wherein the anti-sense strand is complementary to a target region, and,
- b. at least two block oligomers, wherein the at least two block oligomers further comprise PNA, wherein the block oligomer can hybridize to the anti-sense strand to form an anti-sense complex;
- delivering the anti-sense complex to a sample comprising the target polynucleotide sequence, and;
- reducing the amount of the target polynucleotide sequence.
A method of reducing the amount of at least one target polynucleotide sequence comprising,
- providing,
- i. an anti-sense strand, wherein the anti-sense strand is complementary to a target region, and,
- ii. a combination oligomer, wherein the combination oligomer further comprises a PNA nucleobase sequence, a first RNA nucleobase sequence, and a second RNA nucleobase sequence, wherein the combination oligomer hybridizes to the anti-sense strand, to form an anti-sense complex,
- delivering the anti-sense complex to a sample comprising the target polynucleotide sequence, and;
- reducing the amount of the target polynucleotide sequence.
A composition of matter comprising,
- a. an anti-sense strand complementary to a target region, and,
- b. a first block oligomer, a second block oligomer, and a third block oligomer, wherein the first block oligomer and the third block oligomer each comprise an RNA nucleobase sequence, and the second block oligomer comprises a PNA nucleobase sequence, wherein the first block oligomer, the second block oligomer, and the third block oligomer are hybridized to the anti-sense strand.
A kit for reducing the amount of a target polynucleotide sequence comprising,
- a means for transfecting, and,
- an anti-sense complex.
A kit for reducing the amount of a target polynucleotide sequence comprising,
- an anti-sense complex, and,
- a transfection agent.
Dependencies are inferred from the claim text.