TMS1 compositions and methods of use
AssigneeEmory University
InventorPaula Vertino
Life of this patent
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Abstract
The invention relates to the identification of a novel gene, TMS1, which is transcriptionally silenced as a result of methylation. Nucleic acids and polypeptides are provided as are methods and tools for diagnosing and treating disorders characterized by such methylation, and/or abnormally low levels of TMS1 expression products.
Claims22
1-4. (canceled)
A method for determining a risk of developing a disorder characterized by abnormal methylation of a CpG island containing TMS1 nucleic acid molecule comprising measuring a level of an expression product of a CpG island containing TMS1 nucleic acid molecule, by contacting a biological sample isolated from a subject with an agent that selectively binds to a target expression product selected from the group consisting of
- (a) nucleic acid expression products which hybridize under stringent conditions to a complement of a molecule comprising a nucleotide sequence selected from the group consisting of SEQ ID NO:2 and SEQ ID NO:24 and which code for a native TMS1 polypeptide, and
- (b) polypeptide expression products, and fragments thereof, of (a), determining a level of interaction between the agent and the target expression product, and
- comparing the level of interaction between the agent and the target expression product with a control.
6-67. (canceled)
An isolated nucleic acid molecule, comprising
- (a) nucleic acid molecules which hybridize under stringent conditions to a complement of a molecule having a nucleotide sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:20, SEQ ID NO:22 and SEQ ID NO:24, and which code for a native TMS1 polypeptide,
- (b) deletions, additions and substitutions of (a), which code for an apoptosis inducing polypeptide,
- (c) nucleic acid molecules that differ from the nucleic acid molecules of (a) or (b) in codon sequence due to the degeneracy of the genetic code, and
- (d) complements of (a), (b) or (c).
An isolated nucleic acid molecule comprising a nucleotide sequence of SEQ ID NO:4.
An isolated nucleic acid molecule selected from the group consisting of
- (a) a unique fragment of a nucleic acid molecule comprising a nucleotide sequence of SEQ ID NO:1, and
- (b) complements of (a)
- provided that the unique fragment includes a sequence of contiguous nucleotides which is not identical to any sequence from the sequence group consisting of
- (1) sequences having the database accession numbers of Table 1, and
- (2) complements of (1).
86-109. (canceled)
Dependencies are inferred from the claim text.