Primers for isothermal amplification of hepatitis C virus
OwnerMaiko Tanabe, Chihiro Uematsuinventors
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Abstract
The present application relates to primers for isothermal amplification of HCV each include at least eighteen consecutive bases corresponding to a 3′ end region of one selected from base sequences of SEQ ID NOs: 1-10, 21 and 22. The primers are specific to HCV subtypes 1a, 1b, 2a, 2b and 3a, respectively and enable genotyping of HCV by isothermal amplification.
Claims9
A primer set for isothermal amplification of hepatitis C virus containing at least one pair of primers each of which comprises at least eighteen consecutive bases corresponding to a 3′ end region of one base sequence selected from the group consisting of SEQ ID NOs:
- 1-10, 21 and 22.
A method for detecting hepatitis C virus, comprising the steps of:
- subjecting a clinical sample to isothermal amplification with at least one pair of primers selected from the following pairs of primers (1) to (5);
- and
- determining a subtype of hepatitis C virus based on the resulting amplified product:
- (1) a pair of primers comprising at least eighteen consecutive bases corresponding to a 3′ end region of the base sequences of SEQ ID NOs:
- 1 and 2, respectively;
- (2) a pair of primers comprising at least eighteen consecutive bases corresponding to a 3′ end region of the base sequences of SEQ ID NOs:
- 3 and 4, respectively, or a pair of primers comprising at least eighteen consecutive bases corresponding to a 3′ end region of the base sequences of SEQ ID NOs:
- 3 and 9, respectively;
- (3) a pair of primers comprising at least eighteen consecutive bases corresponding to a 3′ end region of the base sequences of SEQ ID NOs:
- 5 and 6, respectively, or a pair of primers comprising at least eighteen consecutive bases corresponding to a 3′ end region of the base sequences of SEQ ID NOs:
- 5 and 10, respectively;
- (4) a pair of primers comprising at least eighteen consecutive bases corresponding to a 3′ end region of the base sequences of SEQ ID NOs:
- 7 and 8, respectively;
- and
- (5) a pair of primers comprising at least eighteen consecutive bases corresponding to a 3′ end region of the base sequences of SEQ ID NOs:
- 21 and 22, respectively.
A kit for detecting hepatitis C virus, comprising at least one pair of primers selected from the following pairs of primers (1) to (5);
- and
- at least one probe containing a sequence complementary to an amplified product amplified by the action of the at least one pair of primers and serving to detect the amplified product:
- (1) a pair of primers comprising at least eighteen consecutive bases corresponding to a 3′ end region of the base sequences of SEQ ID NOs:
- 1 and 2, respectively;
- (2) a pair of primers comprising at least eighteen consecutive bases corresponding to a 3′ end region of the base sequences of SEQ ID NOs:
- 3 and 4, respectively, or a pair of primers comprising at least eighteen consecutive bases corresponding to a 3′ end region of the base sequences of SEQ ID NOs:
- 3 and 9, respectively;
- (3) a pair of primers comprising at least eighteen consecutive bases corresponding to a 3′ end region of the base sequences of SEQ ID NOs:
- 5 and 6, respectively, or a pair of primers comprising at least eighteen consecutive bases corresponding to a 3′ end region of the base sequences of SEQ ID NOs:
- 5 and 10, respectively;
- (4) a pair of primers comprising at least eighteen consecutive bases corresponding to a 3′ end region of the base sequences of SEQ ID NOs:
- 7 and 8, respectively;
- and
- (5) a pair of primers comprising at least eighteen consecutive bases corresponding to a 3′ end region of the base sequences of SEQ ID NOs:
- 21 and 22, respectively.
Dependencies are inferred from the claim text.