Determination of testosterone by mass spectrometry
AssigneeQuest Diagnostics Incorporated
InventorsMichael Caulfield, Darren Carns, Richard Reitz
Life of this patent
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Abstract
Provided are methods for determining the presence or amount of testosterone in a test sample, comprising ionizing all or a portion of the testosterone present in the sample to produce one or more testosterone ions that are detectable in a mass spectrometer. All or a portion of the testosterone present in the sample is ionized to produce one or more testosterone ions, which may be isolated and fragmented to produce precursor ions. A separately detectable internal testosterone standard can be provided in the sample. In a preferred embodiment, the reference is 2, 2, 4, 6, 6-d5 testosterone.
Claims58
A method for determining the presence or amount of testosterone in a test sample comprising,
- (a) purifying testosterone from the test sample by chromatography;
- (b) ionizing the purified testosterone to produce one or more testosterone ions detectable by a mass spectrometer having a mass/charge ratio selected from the group consisting of 289.1±0.5, 109.2±0.5, and 96.9±0.5;
- and
- (c) detecting the presence or amount of the testosterone ion(s) by a mass spectrometer, wherein the presence or amount of the testosterone ion(s) is related to the presence or amount of testosterone in the test sample, wherein purification is achieved using a chromatography system which is connected in-line to the mass spectrometer.
A method for determining the presence or amount of testosterone in a test sample comprising,
- (a) purifying testosterone from the test sample by turbulent flow chromatography;
- (b) ionizing the purified testosterone to produce one or more testosterone ions detectable by a mass spectrometer having a mass/charge ratio selected from the group consisting of 289.1±0.5, 109.2±0.5, and 96.9±0.5;
- and
- (c) detecting the presence or amount of the testosterone ion(s) by a mass spectrometer, wherein the presence or amount of the testosterone ion(s) is related to the presence or amount of testosterone in the test sample.
Dependencies are inferred from the claim text.