Method for identifying bronchoconstriction relaxing substances
OwnerStaffan Skogvallinventor
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Abstract
In a method of measuring a relaxing effect on constricted human bronchi of a candidate substance a bronchus tissue preparation is mounted to a force transducer in a test apparatus. After conditioning the preparation is exposed for a contraction-effective dose of a known contraction-effective substance to make it assume a first tensioned state. The preparation is then exposed for a dose of the candidate substance to make it assume a second tensioned state. By comparing the contraction forces recorded for said tensioned states, a measure of the bronchorelaxing efficiency of the candidate substance is obtained. Also disclosed is a candidate substance thus identified, its uses, and a corresponding bronchus tissue preparation.
Claims20
A method of measuring a bronchorelaxing effect of a candidate substance on constricted human bronchi, comprising
- (a) providing an apparatus for determining the contractile state of a bronchus tissue preparation having the preparation immersed in a physiological medium mounted therein, the apparatus comprising a force transducer fixed to the preparation;
- (b) establishing a substantially non-tensioned base line state of the preparation;
- (c) exposing the preparation to a contraction-effective dose of a contraction-effective agent to make it assume a first tensioned state;
- (d) exposing the preparation to the candidate substance;
- (e) allowing the preparation to return to a base line state;
- (f) repeating step (c) to make the preparation assume a second tensioned state;
- and
- (g) comparing the contraction maxima from the respective tensioned state in steps (c) and (f) to the baseline state to obtain a measure of the bronchorelaxing efficiency of the candidate substance.
Dependencies are inferred from the claim text.