Neutral phenol oxidase
OwnerYoshio Tsujino, Katsunori Endo +2inventors
InventorsYoshio Tsujino, Katsunori Endo, Abul Khaer Mohamad Quamrul Hasan, Kaori Otsuka
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Abstract
The present invention relates to a neutral phenol oxidase showing small changes depending on a substrate in optimum pH for various substrates and having high enzymatic activity at pH around neutral region, a method for producing the same, and an antibody raised against the neutral phenol oxidase. According to the present invention, dyeing of fibers, coloring of hair or the like can be achieved. In addition, according to the present invention, effects on the environment, the human body, and the like can be reduced.
Claims11
A neutral phenol oxidase having the following properties:
- (1) optimum pH:
- being from 5.0 to 7.0, and
- (2) substrate specificity:
- i) catalyzing a coloring reaction by an oxidation of each of N,N-dimethyl-para-phenylenediamine, ortho-aminophenol, 2,6-dimethoxyphenol, 1,3-dihydroxynaphthol, and 4-hydroxyindole at a pH of around 6.5;
- and
- ii) catalyzing an oxidative polymerization reaction of alkali extract of lignin.
A production method of a neutral phenol oxidase, characterized in that a basidiomycete belonging to the genus Flammulina is cultured at a pH of 6.0 to 12.0.
Dependencies are inferred from the claim text.