Method for measuring polyamines in erythrocytes, diagnostic method and kit for measuring polyamines in erythrocytes
AssigneeNational University Corporation Nagoya University
InventorKenji Takagi
Life of this patent
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Abstract
A method for measuring polyamines in erythrocytes in which a polyamine oxidase having a substrate specificity to spermine and spermidine or a polyamine oxidase having a substrate specificity to spermine only is used, an eluate in erythrocytes separated and purified from blood is reacted with these enzymes, and hydrogen peroxide formed is determined with a highly sensitive chromogen such as a diphenylamine-based chromogen. There is provided a method for measuring amounts of polyamines in erythrocytes easily with high precision. The method is effective for diagnosis of certain disease conditions or physiological conditions or the like of subjects.
Claims20
A method for measuring polyamines in erythrocytes, comprising
- a step of reacting an eluate of erythrocytes separated and purified from blood with a polyamine oxidase having a substrate specificity to spermine and spermidine, and
- a step of determining hydrogen peroxide formed in the foregoing step with a highly sensitive chromogen to measure a total amount of spermine and spermidine in erythrocytes.
A method for measuring polyamines in erythrocytes, comprising
- a step of reacting an eluate of erythrocytes separated and purified from blood with a polyamine oxidase having a substrate specificity to spermine, and
- a step of determining hydrogen peroxide formed in the foregoing step with a highly sensitive chromogen to measure an amount of spermine in erythrocytes.
A kit for measuring polyamines in erythrocytes, comprising
- a polyamine oxidase having a substrate specificity to spermine and spermidine, and
- a diphenylamine-based highly sensitive chromogen for determining hydrogen peroxide,
- wherein the kit is used for measuring a total amount of spermine and spermidine in erythrocytes.
A kit for measuring polyamines in erythrocytes, comprising
- a polyamine oxidase having a substrate specificity to spermine, and
- a diphenylamine-based highly sensitive chromogen for determining hydrogen peroxide,
- wherein the kit is used for measuring an amount of spermine in erythrocytes.
A kit for measuring polyamines in erythrocytes, comprising
- a polyamine oxidase having a substrate specificity to spermine and spermidine,
- a polyamine oxidase having a substrate specificity to spermine, and
- a diphenylamine-based highly sensitive chromogen for determining hydrogen peroxide,
- wherein the kit is used for calculating spermidine in erythrocytes.
Dependencies are inferred from the claim text.