Looped probe design to control hybridization stringency
OwnerSukanta Banerjee, Michael Seul +4inventors
InventorsSukanta Banerjee, Michael Seul, Alice Li, Kairali Podual, Chiu Chau, Zhi Li
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Abstract
The present invention provides a method for the generation of novel libraries of encoded magnetic particles from sub-libraries of by the generation of novel sub- libraries of magnetic nanoparticles and encoded particles. The sub-libraries are functionalized on demand are useful in the formation of arrays. The present invention is especially useful for performing multiplexed (parallel) assays for qualitative and/or quantitative analysis of binding interactions of a number of analyte molecules in a sample.
Claims55
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A method of controlling molecular stringency in the hybridization of target nucleic acid sequences and a probe, wherein hybridization is detected to identify the presence of said target nucleic acid sequence in the sample, comprising:
- providing a probe having a first segment complementary with at least a portion of said target nucleic acid sequences and a second segment, separated from said first segment by a linker segment complementary to said first segment;
- and
- controlling which target nucleic acid sequences in a sample hybridize to the first segment or to the second segment by selecting the length of the second segment such that the stability of a duplex formed by first and the second segment is adjusted to intentionally reduce the number of different target nucleic acid sequences which will hybridize to the probe during the assay.
A method of assaying for a target nucleic acid sequence in a sample wherein target nucleic acid sequences in a sample hybridize with a probe and the hybridization is detected to identify the presence of said target nucleic acid sequences in the sample, comprising:
- providing a probe having a first segment complementary with at least a portion of said target nucleic acid sequences and a second segment, separated from said first segment with a linker segment, which is complementary to said first segment;
- placing the probe and the sample in contact under conditions wherein the second segment and the target nucleic acid segment competitively hybridize to the first segment;
- and detecting hybridization of the target nucleic acid segment to the first segment.
An oligonucleotide for use in assays for target nucleic acid sequences, comprising a first segment complementary with at least a portion of said target nucleic acid sequences and a second segment, separated from said first segment with a linker segment, which is complementary to and capable of hybridizing with said first segment.
Dependencies are inferred from the claim text.