Methods and compositions for optimization of oxygen transport by cell-free systems
OwnerRobert Winslowinventor
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Abstract
Compositions, and methods of use thereof, for use as blood substitute products comprise aqueous mixtures of oxygen-carrying and non-oxygen carrying plasma expanders and methods for the use thereof. The oxygen-carrying component may consist of any hemoglobin-based oxygen carrier, while the non-oxygen carrying plasma expander my consist of any suitable diluent.
Claims15
1-44. (canceled)
An aqueous cell-free composition for administration to a subject, comprising polyalkylene oxide modified hemoglobin in an aqueous solution, wherein said modified hemoglobin has a molecular radius larger than native hemoglobin, wherein said composition has a viscosity at least half that of blood, and an oncotic pressure higher than that of plasma, and wherein said polyalkylene oxide is covalently attached to cysteine residues on the hemoglobin.
An aqueous cell-free composition for administration to a subject, comprising polyethylene glycol conjugated to sulfhydryl groups on human hemoglobin to form a modified hemoglobin in an aqueous solution, wherein said modified hemoglobin has a molecular radius larger than native hemoglobin, wherein said composition has a viscosity from 2 to 4.5 cPs, an oncotic pressure from 20 to 60 mm Hg, and a modified hemoglobin concentration greater than 4.0 g/dl.
Dependencies are inferred from the claim text.