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US 2005/0239209 A1Published application
Method for peptide and polypeptide purification and differential analysis
OwnerJonathan Krakover, Ramila Philipinventors
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OwnerJonathan Krakover, Ramila Philipinventors
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A method for peptide and polypeptide purification that uses a derivatized solid support to capture all of the polypeptides and peptides present in a solution and in a subsequent step quantitatively release all of the polypeptides and peptides bound to the solid support is disclosed. This method provides for quantitative recovery and permits further analysis by any of a variety of analytical methods, including sequencing, chromatography, mass spectrometry and biological assay. The method may further be used for screening peptides and polypeptides that bind to organic molecules such as small molecule drugs. The method does not irreversibly add any unwanted label moieties or otherwise alter the peptides during the release process that could interfere with subsequent processing or analysis steps, in particular, biological assay to assess function in vivo or in vitro in cell-based, whole animal or human testing.
1) A method for the purification and quantitative recovery of a polypeptide of length n having a free amino terminus comprising the steps of:
2) A method of labeling a polypeptide of length n having an amino terminus comprising the steps of:
3) A method for identification of MHC-associated polypeptide targets of a compound, comprising:
Dependencies are inferred from the claim text.