Gb virus b based replicons and replicon enhanced cells
OwnerAmedeo De Tomassi, Rita Graziani +2inventors
InventorsAmedeo De Tomassi, Rita Graziani, Giacomo Paonessa, Cinzia Traboni
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Abstract
The present invention features GBV-B replicons and replicon enhanced cells. A GBV-B replicon is an RNA molecule able to autonomously replicate in a cultured cell and produce detectable levels of one or more GBV-B proteins. GBV-B replicon enhanced cells are cells having an increased ability to maintain a GBV-B replicon.
Claims25
A GBV-B replicon comprising the following regions:
- a GBV-B 5′ UTR substantially similar to bases 1-445 of SEQ ID NO 1;
- a selection or reporter sequence functionally coupled to said GBV-B 5′ UTR;
- an internal ribosome entry site;
- a NS3-NS5B sequence substantially similar to bases 1938-7709 of SEQ ID NO:
- 1 functionally coupled to said internal ribosome entry site and an AUG translation initiation codon;
- and
- a GBV-B 3′ UTR substantially similar to bases 7710-8069 of SEQ ID NO:
- 1,
- wherein said replicon is capable of replication in a cell.
A GBV-B replicon enhanced cell, wherein said cell has an maintenance and activity efficiency of at least 25% when transfected with a GBV-B replicon of SEQ ID NO:
- 1 by the Electroporation Method.
21-23. (canceled)
25-26. (canceled)
28-47. (canceled)
Dependencies are inferred from the claim text.